Guinea pigs were sensitized with trinitrophenylated liver macromolecular protein fraction
(TNP-LP1) prepared by using sodium trinitrobenzenesulfonate of strong immunogenicity
as the hapten and LP1 as the carrier protein. The administration of trinitrophenylated
hepatocytes and lipopolysaccharide to these TNP-LP1-sensitized guinea pigs through
the mesenteric vein 2 weeks later resulted in the induction of acute hepatic failure
accompanied by massive hepatic cell necrosis in almost all of the guinea pigs. Using
this experimental model, the effect of Gomisin A on the induction of immunological
acute hepatic failure was examined. As a result, the administration of gomisin A remarkably
improved the survival rate and serum transaminase levels of the immunologically-induced
acute hepatic failure guinea pigs. Gomisin A also improved the histological changes
of the liver in these guinea pigs. These results suggested that gomisin A is effective
for the improvement of immunologically-induced acute hepatic failure in our experimental
model.
Gomisin A - hepatic failure model - hepatoprotective action - immunological mechanisms
- experimental model
